Sonics VCX750 (Serial No."Y through "AB")& Bedienungsanleitung

U S E R ’ S G U I D EAUTOTUNE SERIESHIGH INTENSITY ULTRASONIC PROCESSORWITH TEMPERATURE CONTROLLERMICROPROCESSOR CONTROLLED (SERIAL NO. "Y"

7No DESCRIPTIONOrderNumber1 Converter Model CV33 CV000332 Four element coupler 630-05583 Stepped top(s) 1/8” (3mm) 630-05354 Booster BHNVCGD5 Probe ½”

8USING THE ULTRASONIC PROCESSORThe speed control on an automobile, can, to a certain extent, be compared to anUltrasonic Processor. The speed control

Press the I key. The screen will display the power rating and the frequency of theUltrasonic Processor, and the following control parameters.AMPLITUDE

1. Using the fittings on top of the converter, circulate dry compressed air through theconverter to cool the converter.2. Immerse the probe into the l

3. Depress the START key.4. Using the▲ or ▼ keys, increase or decrease the amplitude as required.TIMER: In the pulsed mode the processing time will be

PULSER: By inhibiting heat build-up in the sample, the pulse function enables safetreatment of temperature sensitive samples at high intensity. In add

TEMPERATURE: The temperature function prevents overheating of the sample bycontinuously monitoring the sample temperature, and terminating the ultraso

14REVIEW: The REVIEW function provides a “window” on the process by displayingvarious operating parameters without process interruption. Pressing theE

ENERGY SETPOINT: The energy setpoint continuously monitors the amount ofenergy in Joules (watts-seconds), that is being delivered to the probe, and te

RECALL: The recall function can retrieve from memory any of 9 stored controlparameters for verification or usage. To retrieve any parameters press the

WARRANTYYour Ultrasonic Processor is warranted for a period of three years from thedate of shipment against defects in material and workmanship under

SECTION III – SERVICE INFORMATIONYour Ultrasonic Processor was designed to provide you with years of safe anddependable service. Nevertheless, because

18RETURN OF EQUIPMENTIt is suggested that if a unit is in need of repair, it should be sent back to the factory.In order to receive prompt service; al

19SONICS SAFETY CERTIFICATION FORMItems being returned:Please check only one item below:___ The equipment was never used or exposed to any radiologica

20SECTION IV -OPERATING SUGGESTIONS AND TECHNIQUESDISRUPTING CELLSThe disruption of cells is an important method in the field of proteomics and in the

21Single-cell organisms (micro-organisms) consist of a semipermeable, tough, rigidouter cell wall surrounding the protoplasmic membrane and cytoplasm.

22The ability to control the amplitude at the probe tip is a prerequisite for processoptimization. And because each application requires its own set o

23the sample remains trapped in intact cells and, therefore, is unavailable forsubsequent purification. For most samples, thorough disruption can be m

24Detergent cell lysis is sometimes used in conjunction with ultrasonic processing tofacilitate disruption. Detergents break the lipid barrier surroun

25shown to effectively release recombinant proteins located in the cytoplasm of bacteriaand is recommended when lysing mammalian cells. However, this

26When processing a sample with ultrasonics, always immerse the probe deep enoughbelow the surface of the sample to inhibit aerosoling or foaming, foa

IMPORTANT SAFEGUARDSREAD BEFORE INSTALLING ORUSING THE EQUIPMENTYour Ultrasonic Processor has been designed with safety in mind. However, no designcan

27Before each application, place the tip in 100% ethanol and energize the power supplyfor a few seconds to remove any residual substances. If still co

28Fractional protein release, Rp, is calculated using the following equation andmultiplying the result by 100:Rp = Cf – CbCt – CbCf = Free proteinCt =

1SECTION I – INSTALLATIONINSPECTIONPrior to installing the Ultrasonic Processor, perform a visual inspection to detect anyevidence of damage which mig

2SECTION II – OPERATIONPRINCIPLES OF ULTRASONIC DISRUPTIONThe ultrasonic power supply converts 50/60 Hz line voltage to high frequencyelectrical energ

Displays prompts and the following control parameters:• Amplitude selected• Output power delivered to the probe in watts, and as percentage of the tot

FUNCTIONS OF KEYS, CONTROLS, INDICATORS, AND CONNECTORS (cont.)REAR PANEL9-pin D-sub connector Connects to external actuation device, and enable power

PREPARATION FOR USE1. Plug the electrical line cord into the electrical outlet. If the unit is already on, switchthe unit off by depressing the O key.

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